Adrenal chromaffin cells contain functionally different SNAP-25 monomers and SNAP-25/syntaxin heterodimers
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چکیده
منابع مشابه
Snap-25/syntaxin 1a Complex Functionally Modulates Neurotransmitter Gaba Reuptake
NEUROTRANSMITTER GABA REUPTAKE Hua-Ping Fan ‡§, Feng-Juan Fan ‡§, Lan Bao‡ and Gang Pei‡ From the ‡Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and the §Graduate School of Chinese Academy of Sciences; 320 Yue Yang Road, Shanghai 200031, People’s Republic of China. Running Title: SNAP-25...
متن کاملPatch-clamp capacitance analysis of the effects of alpha-SNAP on exocytosis in adrenal chromaffin cells.
We have examined the effect of alpha-SNAP on exocytosis in adrenal chromaffin cells by direct assay of exocytosis using patch-clamp capacitance analysis. Cells were recorded using the whole cell patch-clamp configuration and the cells dialysed with control pipette solution or with a pipette solution containing alpha-SNAP or the deletion mutant alpha-SNAP(41-295). The deletion mutant was found t...
متن کاملTargeting of SNAP-23 and SNAP-25 in polarized epithelial cells.
SNAP-23 is the ubiquitously expressed homologue of the neuronal SNAP-25, which functions in synaptic vesicle fusion. We have investigated the subcellular localization of SNAP-23 in polarized epithelial cells. In hepatocyte-derived HepG2 cells and in Madin-Darby canine kidney (MDCK) cells, the majority of SNAP-23 was present at both the basolateral and apical plasma membrane domains with little ...
متن کاملکلونینگ، بیان و تخلیص پروتئین SNAP-25
Background & Objectives:Clostridial neurotoxin inhibits neurotransmitter release by selective and specific intracellular proteolysis of synaptosomal associated protein of 25KDa (SNAP-25), synaptobrevin/VAMP-2 and syntaxin. SNAP-25 is one of the components that forms docking complex in synaptic ends. This protein is subtrate for botulinum neurotoxins types A,C, and E. Each of these toxin serotyp...
متن کاملDifferential Control of the Releasable Vesicle Pools by SNAP-25 Splice Variants and SNAP-23
The SNARE complex, consisting of synaptobrevin, syntaxin, and SNAP-25, is essential for calcium-triggered exocytosis in neurosecretory cells. Little is known, however, about how developmentally regulated isoforms and other cognate SNARE components regulate vesicular fusion. To address this question, we examined neuroexocytosis from chromaffin cells of Snap25 null mice rescued by the two splice ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1996
ISSN: 0014-5793
DOI: 10.1016/0014-5793(96)00931-3